from Journal of the American Society of Nephrology current issue https://ift.tt/2rbO3...
Monday, April 30, 2018
JASN this Month: Something Old, Something New
from Journal of the American Society of Nephrology current issue https://ift.tt/2w0dK...
Repairing the GBM Step by Step
from Journal of the American Society of Nephrology current issue https://ift.tt/2vZ6y...
WNKs on the Fly
from Journal of the American Society of Nephrology current issue https://ift.tt/2vWYc...
Insights into CKD from Metabolite GWAS
from Journal of the American Society of Nephrology current issue https://ift.tt/2vZ6f...
Using Large Datasets to Understand CKD
from Journal of the American Society of Nephrology current issue https://ift.tt/2vZ67...
The Quest for Better Biomarkers of Bone Turnover in CKD
from Journal of the American Society of Nephrology current issue https://ift.tt/2vZ5R...
HDL in CKD--The Devil Is in the Detail
The picture of HDL cholesterol (HDL-C) as the "good" cholesterol has eroded. This is even more surprising because there exists strong evidence that HDL-C is associated with cardiovascular disease (CVD) in the general population as well as in patients with impairment of kidney function and/or progression of CKD. However, drugs that dramatically increase HDL-C have mostly failed to decrease CVD events. Furthermore, genetic studies took the same line,...
The Ebb and Flow of Echocardiographic Cardiac Function Parameters in Relationship to Hemodialysis Treatment in Patients with ESRD
Cardiovascular disease is the leading cause of mortality in patients receiving hemodialysis. Cardiovascular events in these patients demonstrate a day-of-week pattern; i.e., they occur more commonly during the last day of the long interdialytic interval and the first session of the week. The hemodialysis process causes acute decreases in cardiac chamber size and pulmonary circulation loading and acute diastolic dysfunction, possibly through myocardial...
Protein S Protects against Podocyte Injury in Diabetic Nephropathy
Background Diabetic nephropathy (DN) is a leading cause of ESRD in the United States, but the molecular mechanisms mediating the early stages of DN are unclear.
Methods To assess global changes that occur in early diabetic kidneys and to identify proteins potentially involved in pathogenic pathways in DN progression, we performed proteomic analysis of diabetic and nondiabetic rat glomeruli. Protein S (PS) among the highly upregulated proteins in...
Mechanism of Hyperkalemia-Induced Metabolic Acidosis
Background Hyperkalemia in association with metabolic acidosis that are out of proportion to changes in glomerular filtration rate defines type 4 renal tubular acidosis (RTA), the most common RTA observed, but the molecular mechanisms underlying the associated metabolic acidosis are incompletely understood. We sought to determine whether hyperkalemia directly causes metabolic acidosis and, if so, the mechanisms through which this occurs.
Methods...
Laminin-521 Protein Therapy for Glomerular Basement Membrane and Podocyte Abnormalities in a Model of Pierson Syndrome
Background Laminin α5β21 (LM-521) is a major component of the GBM. Mutations in LAMB2 that prevent LM-521 synthesis and/or secretion cause Pierson syndrome, a rare congenital nephrotic syndrome with diffuse mesangial sclerosis and ocular and neurologic defects. Because the GBM is uniquely accessible to plasma, which permeates endothelial cell fenestrae, we hypothesized that intravenous delivery of LM-521 could replace the missing LM-521 in the GBM...
Lipoxins Regulate the Early Growth Response-1 Network and Reverse Diabetic Kidney Disease
Background The failure of spontaneous resolution underlies chronic inflammatory conditions, including microvascular complications of diabetes such as diabetic kidney disease. The identification of endogenously generated molecules that promote the physiologic resolution of inflammation suggests that these bioactions may have therapeutic potential in the context of chronic inflammation. Lipoxins (LXs) are lipid mediators that promote the resolution...
Intracellular Chloride and Scaffold Protein Mo25 Cooperatively Regulate Transepithelial Ion Transport through WNK Signaling in the Malpighian Tubule
Background With No Lysine kinase (WNK) signaling regulates mammalian renal epithelial ion transport to maintain electrolyte and BP homeostasis. Our previous studies showed a conserved role for WNK in the regulation of transepithelial ion transport in the Drosophila Malpighian tubule.
Methods Using in vitro assays and transgenic Drosophila lines, we examined two potential WNK regulators, chloride ion and the scaffold protein mouse protein 25 (Mo25),...
Nephrin-Binding Ephrin-B1 at the Slit Diaphragm Controls Podocyte Function through the JNK Pathway
Background B-type ephrins are membrane-bound proteins that maintain tissue function in several organs. We previously reported that ephrin-B1 is localized at the slit diaphragm of glomerular podocytes. However, the function of ephrin-B1 at this location is unclear.
Methods We analyzed the phenotype of podocyte-specific ephrin-B1 knockout mice and assessed the molecular association of ephrin-B1 and nephrin, a key molecule of the slit diaphragm, in...
Gpr97 Exacerbates AKI by Mediating Sema3A Signaling
Background G protein-coupled receptors (GPCRs) participate in a variety of physiologic functions, and several GPCRs have critical physiologic and pathophysiologic roles in the regulation of renal function. We investigated the role of Gpr97, a newly identified member of the adhesion GPCR family, in AKI.
Methods AKI was induced by ischemia–reperfusion or cisplatin treatment in Gpr97-deficient mice. We assessed renal injury in these models and in patients...
Genome-Wide Mapping of DNA Accessibility and Binding Sites for CREB and C/EBP{beta} in Vasopressin-Sensitive Collecting Duct Cells
Background Renal water excretion is controlled by vasopressin, in part through regulation of the transcription of the aquaporin-2 gene (Aqp2).
Methods To identify enhancer regions likely to be involved in the regulation of Aqp2 and other principal cell–specific genes, we used several next generation DNA-sequencing techniques in a well characterized cultured cell model of collecting duct principal cells (mpkCCD). To locate enhancers, we performed...
Similar Biophysical Abnormalities in Glomeruli and Podocytes from Two Distinct Models
Background FSGS is a pattern of podocyte injury that leads to loss of glomerular function. Podocytes support other podocytes and glomerular capillary structure, oppose hemodynamic forces, form the slit diaphragm, and have mechanical properties that permit these functions. However, the biophysical characteristics of glomeruli and podocytes in disease remain unclear.
Methods Using microindentation, atomic force microscopy, immunofluorescence microscopy,...
Genome-Wide Association Studies of Metabolites in Patients with CKD Identify Multiple Loci and Illuminate Tubular Transport Mechanisms
Background The kidneys have a central role in the generation, turnover, transport, and excretion of metabolites, and these functions can be altered in CKD. Genetic studies of metabolite concentrations can identify proteins performing these functions.
Methods We conducted genome-wide association studies and aggregate rare variant tests of the concentrations of 139 serum metabolites and 41 urine metabolites, as well as their pairwise ratios and fractional...
Characterization of Coding/Noncoding Variants for SHROOM3 in Patients with CKD
Background Interpreting genetic variants is one of the greatest challenges impeding analysis of rapidly increasing volumes of genomic data from patients. For example, SHROOM3 is an associated risk gene for CKD, yet causative mechanism(s) of SHROOM3 allele(s) are unknown.
Methods We used our analytic pipeline that integrates genetic, computational, biochemical, CRISPR/Cas9 editing, molecular, and physiologic data to characterize coding and noncoding...
The Most N-Terminal Region of THSD7A Is the Predominant Target for Autoimmunity in THSD7A-Associated Membranous Nephropathy
Background Thrombospondin type 1 domain–containing 7A (THSD7A) has been identified as a pathogenic autoantigen in membranous nephropathy (MN). However, the THSD7A epitopes targeted by patient autoantibodies are unknown.
Methods We performed an in silico analysis of the THSD7A multidomain structure, expressed the folded domains in HEK293 cells, and tested for domain reactivity with 31 serum samples from patients with THSD7A-associated MN using Western...
Biomarkers of AKI Progression after Pediatric Cardiac Surgery
Background As children progress to higher stages of AKI, the risk for adverse outcomes dramatically increases. No reliable methods exist to predict AKI progression in hospitalized children. To determine if biomarkers of inflammation and kidney injury can predict AKI progression, we conducted a three-center prospective cohort study of children undergoing cardiopulmonary bypass.
Methods On the first day of serum creatinine–defined AKI, we measured...
Diagnostic Accuracy of Biomarkers and Imaging for Bone Turnover in Renal Osteodystrophy
Background Renal osteodystrophy is common in advanced CKD, but characterization of bone turnover status can only be achieved by histomorphometric analysis of bone biopsy specimens (gold standard test). We tested whether bone biomarkers and high-resolution peripheral computed tomography (HR-pQCT) parameters can predict bone turnover status determined by histomorphometry.
Methods We obtained fasting blood samples from 69 patients with CKD stages 4–5,...
Ischemia-Induced DNA Hypermethylation during Kidney Transplant Predicts Chronic Allograft Injury
Background Ischemia during kidney transplant causes chronic allograft injury and adversely affects outcome, but the underlying mechanisms are incompletely understood. In tumors, oxygen shortage reduces the DNA demethylating activity of the ten-11 translocation (TET) enzymes, yielding hypermethylated genomes that promote tumor progression. We investigated whether ischemia similarly induces DNA hypermethylation in kidney transplants and contributes...
Antibodies Can Extenuate Polyomavirus Infections
from Journal of the American Society of Nephrology current issue https://ift.tt/2I2mr...
Studying the Effect of Vitamin D Supplementation on Vascular Function in CKD: A Work in Progress
from Journal of the American Society of Nephrology current issue https://ift.tt/2HCit...
The Authors Reply
from Journal of the American Society of Nephrology current issue https://ift.tt/2I2mL...
The Authors Reply
from Journal of the American Society of Nephrology current issue https://ift.tt/2rdq1...
C.D.C. Director’s $375,000 Salary Will Be Cut
By PAM BELLUCK from NYT Health https://ift.tt/2rbsp...
FOX NEWS: Court sides with mom to keep boy on life support as she desperately seeks transfer
Court sides with mom to keep boy on life support as she desperately seeks transfer
In New Jersey, the law determining if a patient is brain dead is not limited to neurological criteri...
FOX NEWS: Mom seeks to move boy declared brain-dead at Pennsylvania hospital to facility in NJ
Mom seeks to move boy declared brain-dead at Pennsylvania hospital to facility in NJ
In New Jersey, the law determining if a patient is brain dead is not limited to neurological criteri...
FOX NEWS: 'Grandma Cuddler' soothes NICU babies at New York hospital
'Grandma Cuddler' soothes NICU babies at New York hospital
A woman dubbed “Grandma Cuddler” isn’t your typical volunteer in the neonatal intensive care unit at NewYork-Presbyterian Morgan Stanley Children’s Hospita...
FOX NEWS: Girl battling bone cancer has leg reattached backward
Girl battling bone cancer has leg reattached backward
When Amelia Eldred, an active 7-year-old in England, needed her leg amputated to treat bone cancer, doctors performed an unusual surgery: They removed the middle part of her leg and then reattached the lower part of her leg — but backwar...
Wheelchairs Prohibited in the Last Place You’d Expect
By PAULA SPAN from NYT Health https://ift.tt/2rgfk...
FOX NEWS: 'So many miracles': Surviving siblings of car crash that killed parents, baby begin road to recovery
'So many miracles': Surviving siblings of car crash that killed parents, baby begin road to recovery
The great aunt of four Texas siblings who lost their 2-month-old sister and both parents in a horrific car crash earlier this month said they have made some improvements, but it’s going to be a long road to recover...
Sunday, April 29, 2018
Injecting Drugs Can Ruin a Heart. How Many Second Chances Should a User Get?
By ABBY GOODNOUGH from NYT Health https://ift.tt/2I0Wv...
Saturday, April 28, 2018
FOX NEWS: Two Texas siblings reunited after car crash that killed parents, baby sister
Two Texas siblings reunited after car crash that killed parents, baby sister
Two San Angelo, Texas, siblings were reunited for the first time after their parents and 2-month-old sister were killed in a head-on crash earlier this mont...
FOX NEWS: Doctors are now 're-gifting' kidneys to save lives
Doctors are now 're-gifting' kidneys to save lives
Experts hope new technique can help with transplanted organ shortag...
FOX NEWS: Alfie Evans, UK toddler at center of legal battle, dies
Alfie Evans, UK toddler at center of legal battle, dies
Alfie Evans, a terminally ill British toddler whose case drew attention from Pope Francis and others around the world, has died, his parents announced Saturday mornin...
Friday, April 27, 2018
The Golden State Killer Is Tracked Through a Thicket of DNA, and Experts Shudder
By GINA KOLATA and HEATHER MURPHY from NYT Health https://ift.tt/2I2fj...
FOX NEWS: Amid romaine lettuce E. coli outbreak, restaurants aim to soothe customers
Amid romaine lettuce E. coli outbreak, restaurants aim to soothe customers
In the weeks since an E. coli bacteria outbreak on romaine lettuce grown in Yuma, Arizona was announced, restaurants and eateries around the country have worked to assure customers their food is saf...
New C.D.C. Director’s $375,000 Salary Under Scrutiny
By SHEILA KAPLAN from NYT Health https://ift.tt/2r5yv...
FOX NEWS: Former model 'eaten alive' while in care of Georgia nursing home
Former model 'eaten alive' while in care of Georgia nursing home
Rebecca Zeni’s family said the former model suffered a terrible death after she was eaten alive by a scabies infestation while in the care of Shepherd Hills Nursing Hom...
FOX NEWS: Mom dies after battling rare rodent-borne virus for weeks
Mom dies after battling rare rodent-borne virus for weeks
A New Mexico family is mourning the loss of a 27-year-old mother who had spent weeks fighting for her life after contracting a rare rodent-borne viru...
FOX NEWS: More kids have autism, better diagnosis may be the reason
More kids have autism, better diagnosis may be the reason
The government estimates that autism is becoming more common, but it's only a small increase and some experts think it can be largely explained by better diagnosing of minority childre...
FOX NEWS: Halsey announces she plans to freeze her eggs due to endometriosis
Halsey announces she plans to freeze her eggs due to endometriosis
Halsey announced she plans to freeze her eggs due to her recent diagnosis of endometriosi...
FOX NEWS: Teacher who smells 'like a fish' due to rare disorder finds relief
Teacher who smells 'like a fish' due to rare disorder finds relief
A mother with an extremely rare condition that makes her smell like fish has finally found relief after years of being humiliated by the odo...
FOX NEWS: Hog farm's neighbors awarded $50M after suing over smells, spraying
Hog farm's neighbors awarded $50M after suing over smells, spraying
Families who live near a hog farm in Bladen County, N.C., have been complaining for decades about bad smells, flies and excessive chemical sprayin...
Thursday, April 26, 2018
Trump’s Doctor Accused of Handing Out Ambien. D.E.A. Calls the Practice Illegal
By GINA KOLATA from NYT Health https://ift.tt/2Fk9j...
FOX NEWS: A doctor who can feel his patient's pain
A doctor who can feel his patient's pain
What if a doctor could physically feel the pain their patients were experiencing? Dr. Joel Salinas, a neurologist and clinical researcher at Massachusetts General Hospital, can do just that. He has a neurological trait called mirror-touch, a medical mystery that causes him to feel the emotional and physical...
FOX NEWS: Woman claims she watched YouTube clips on childbirth before delivering own baby in hotel room during overseas layover
Woman claims she watched YouTube clips on childbirth before delivering own baby in hotel room during overseas layover
A Tennessee woman’s birth story has gone viral after she claimed on Twitter that she gave birth by herself in her hotel room during a layover in Turkey while on her way to German...
FOX NEWS: Mom claims son's 'hidden' unborn twin gave her cancer
Mom claims son's 'hidden' unborn twin gave her cancer
A mother-of-four is claiming that was left fighting for life when she developed cancer stemming from her baby son's hidden unborn twi...
FOX NEWS: Alfie Evans' parents to meet with doctors to discuss taking terminally ill tot home
Alfie Evans' parents to meet with doctors to discuss taking terminally ill tot home
The parents of Alfie Evans plan to meet with his doctors on Thursday to discuss bringing the terminally ill boy hom...
FOX NEWS: Abandoned dog with one eye undergoes incredible transformation
Abandoned dog with one eye undergoes incredible transformation
A dreadlocked dog that was "one big mat" when he was rescued from the streets has undergone a stunning transformation - and is now looking for a hom...
‘Desperation Oncology’: When Patients Are Dying, Some Cancer Doctors Turn to Immunotherapy
By GINA KOLATA from NYT Health https://ift.tt/2Hwgv...
Wednesday, April 25, 2018
FOX NEWS: Missouri pediatrician slams 'selfish' anti-vaxers after measles Facebook post sparks debate
Missouri pediatrician slams 'selfish' anti-vaxers after measles Facebook post sparks debate
A Missouri pediatrician is not planning to apologize for his facility's Facebook post encouraging Kansas City-area residents to be "responsible" and get vacinated amid a measles outbreak in the are...
A Lifesaving Pump for Cancer Patients Is Being Phased Out
By DENISE GRADY and SHEILA KAPLAN from NYT Health https://ift.tt/2HQ1i...
Infant Deaths Fall Sharply in Africa With Routine Antibiotics
By DONALD G. McNEIL Jr. from NYT Health https://ift.tt/2vNkm...
FOX NEWS: Alfie Evans' parents lose attempt to overturn ruling that barred terminally ill British boy from traveling to Rome
Alfie Evans' parents lose attempt to overturn ruling that barred terminally ill British boy from traveling to Rome
Alfie Evans parents' attempt to overturn their appeal was rejected by the Court of Appeal on Wednesda...
FOX NEWS: Florida mom gets 'blood boiling' letter from neighbors regarding 'hefty' teen with autism
Florida mom gets 'blood boiling' letter from neighbors regarding 'hefty' teen with autism
When Leah Solomon's husband forwarded her a letter he found under their front door, the Florida mother was shocke...
FOX NEWS: Mom has foot amputated after discovering cancerous tumor during pedicure
Mom has foot amputated after discovering cancerous tumor during pedicure
A North Carolina mother’s decision to amputate her right foot in hopes of preventing a cancerous tumor from spreading did not come easy, but she said it was her love of life and her kids that ultimately helped her make i...
FOX NEWS: Former classmate donates life-saving kidney to man nearly 50 years after graduating
Former classmate donates life-saving kidney to man nearly 50 years after graduating
A pair of former classmates who hardly knew each other while in school 50 years ago reunited recently, but it wasn’t to reminisce or catch up -- it was for a life-saving transplant that has bonded the pair for lif...
FOX NEWS: 7-year-old boy recovering after 5-organ transplant surgery
7-year-old boy recovering after 5-organ transplant surgery
A seven-year-old boy is one of the youngest patients in Britain to undergo a multi-transplant operation to successfully replace five organ...
FOX NEWS: Chicago baby fitted with world's tiniest mechanical heart valve
Chicago baby fitted with world's tiniest mechanical heart valve
A Chicago baby is thriving after she became one of the first to receive a mechanical heart valv...
FOX NEWS: Alfie Evans' parents to appeal ruling that barred terminally ill British boy from traveling to Rome
Alfie Evans' parents to appeal ruling that barred terminally ill British boy from traveling to Rome
Alfie Evans’ parents will appeal to overturn a ruling Wednesday that barred the terminally ill child from being taken to Rome for car...
FOX NEWS: Alfie Evans' parents to appeal ruling that barred terminally ill British teen from traveling to Rome
Alfie Evans' parents to appeal ruling that barred terminally ill British teen from traveling to Rome
Alfie Evans’ parents will appeal to overturn a ruling Wednesday that barred the terminally ill child from being taken to Rome for car...
Tuesday, April 24, 2018
FOX NEWS: Alfie Evans' dad giving toddler mouth-to-mouth resuscitation to 'keep him alive'
Alfie Evans' dad giving toddler mouth-to-mouth resuscitation to 'keep him alive'
Alfie Evans' parents are tonight giving him mouth-to-mouth resuscitation in a desperate bid to keep him alive after a court ruled he cannot be flown to Italy for treatmen...
With George H.W. Bush Hospitalized, the World Wonders: Is It a Broken Heart?
By GINA KOLATA and BENEDICT CAREY from NYT Health https://ift.tt/2FhjC...
FOX NEWS: Sorority grants World War II vet's final wish to dance with a beautiful woman
Sorority grants World War II vet's final wish to dance with a beautiful woman
A bedridden 92-year-old World War II veteran had a dying wish fulfilled thanks to a Mississippi sorority-- to dance with a beautiful woma...
FOX NEWS: Alfie Evans' family loses appeal to transport terminally ill British toddler to Rome
Alfie Evans' family loses appeal to transport terminally ill British toddler to Rome
The family of Alfie Evans, the terminally ill British toddler who was taken off life support Monday, lost their legal challenge Tuesday to transport the boy to Rom...
FOX NEWS: 1 dead, another recovering after 2 men suffer heart attacks at same train station
1 dead, another recovering after 2 men suffer heart attacks at same train station
Officers called to a medical emergency at an Atlanta train station last week were called back to the same station an hour later, for the same emergency but with a different patien...
FOX NEWS: Alfie Evans, severely ill British toddler, may be transported to Rome for care if appeal succeeds
Alfie Evans, severely ill British toddler, may be transported to Rome for care if appeal succeeds
Alfie Evans, the terminally ill British toddler who was taken off life support Monday, may be transported to Rome for care if a last-ditch appeal succeeds in cour...
FOX NEWS: 'QLaser' TV infomercial doc gets 12 years in prison for selling scam device
'QLaser' TV infomercial doc gets 12 years in prison for selling scam device
A former dentist-turned convicted con-man and his two cohorts were sentenced to prison on Friday for their roles in a multi-million-dollar fraud case that primarily preyed on the elderl...
F.D.A. Cracks Down on Sales of E-Cigarettes to Minors
By KATE ZERNIKE and SHEILA KAPLAN from NYT Health https://ift.tt/2qYcq...
FOX NEWS: Ice pops recalled over possible listeria contamination
Ice pops recalled over possible listeria contamination
A West Virginia-based company has issued a voluntary recall for 3,000 cases of ice pops sold under two different brands over concerns about listeria contaminatio...
FOX NEWS: Veteran diagnosed with skin cancer after dentist spots mark on face
Veteran diagnosed with skin cancer after dentist spots mark on face
A routine trip to the dentist last year may have played a key role in saving an Oregon veteran’s lif...
FOX NEWS: Woman has leg amputated after break wouldn't heal
Woman has leg amputated after break wouldn't heal
A young woman decided to have her leg amputated after a simple stress fracture she suffered walking down a street wouldn't hea...
